POU2F3 Antibody
| Code | Size | Price |
|---|
| PSI-7795-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7795-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 347658964
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
POU2F3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
POU2F3 Antibody: PLA1, OCT11, PLA-1, Epoc-1, OCT-11, OTF-11, Skn-1a, OTF11, PLA1, POU domain, class 2, transcription factor 3, Octamer-binding protein 11, 41923
Application Note:
POU2F3 antibody can be used for detection of POU2F3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
POU2F3, also known as Epoc-1, is a member of a family of POU domain family of transcription factors (1). POU2F3 is expressed primarily in the epidermis and plays a critical role in keratinocyte proliferation and differentiation (1,2). It is a crucial transcription factor that is required for the development of sweet, umami, and bitter, but not sour taste receptor cells (3). POU2F3 is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer (4).
Background References:
- Yukawa K, Yasui T, Yamamoto A, et al. Epoc-1: a POU-domain gene expressed in murine epidermal basal cells and thymic stromal cells. Gene 1993; 133:163-9.
- Cabral A, Fischer DF, Vermeij WP, et al. Distinct functional interactions of human Skn-1 isoforms with Ese-1 during keratinocyte terminal differentiation. J. Biol. Chem. 2003; 278:17792-9.
- Matsumoto I, Ohmoto M, Narukawa M, et al. Skn-1a/Pou2f3 specifies taste receptor cell lineage. Nat. Neurosci. 2011; 14:685-7.
- Zhang Z, Huettner PC, Nguyen L, et al. Aberrant promoter methylation and silencing of the POU2F3 gene in cervical cancer. Oncogene 2006; 25:5436-45.
Buffer:
POU2F3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
POU2F3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human POU2F3.
The immunogen is located within amino acids 370 - 420 of POU2F3.
The immunogen is located within amino acids 370 - 420 of POU2F3.
NCBI Gene ID #:
25833
NCBI Official Name:
POU class 2 homeobox 3
NCBI Official Symbol:
POU2F3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 48 kDa
Observed: 55 kDa
Observed: 55 kDa
Protein Accession #:
NP_001231611
Protein GI Number:
347658964
Purification:
POU2F3 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
POU2F3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist. This antibody will recognize both isoforms. POU2F3 antibody is predicted to not cross-react with other members of the POU domain class 2 family.
Swissprot #:
Q9UKI9
User NOte:
Optimal dilutions for each application to be determined by the researcher.