SOX17 Antibody

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ProSci
Product Code: PSI-7917
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7917-0.02mg0.02mg£150.00
Quantity:
PSI-7917-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 11967991
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
SOX17 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of SOX17 in HepG2 cell lysate with SOX17 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunofluorescence of SOX17 in mouse liver tissue with SOX17 antibody at 20 μg/ml.
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Western blot analysis of SOX17 in HepG2 cell lysate with SOX17 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunofluorescence of SOX17 in mouse liver tissue with SOX17 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
SOX17 Antibody: VUR3, Transcription factor SOX-17
Application Note:
SOX17 antibody can be used for detection of SOX17 by Western blot at 0.5 - 1 μg/ml. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SOX17 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate (1,2). SOX17 is part of the SoxF subgroup which plays an important role in the differentiation of different cell types, including visceral and definitive endoderm and hematopoietic cell types (3,4). SOX17 is also essential for acquisition and maintenance of arterial identity (5).
Background References:
  • Kanai Y, Kanai-Azuma M, Noce T, et al. Identification of two Sox17 messenger RNA isoforms, with and without the high mobility group box region, and their differential expression in mouse spermatogenesis. J. Cell Biol. 1996; 133:667-81.
  • Jiang T, Hou CC, She ZY, et al. The SOX gene family: function and regulation in testis determination and male fertility maintenance. Mol. Biol. Rep. 2013; 40:2187-94.
  • Francois M, Koopman P, and Beltrame M. SoxF genes: key players in the development of the cardiovascular system. Int. J. Biochem. Cell Biol. 2010; 42:445-8.
  • Kim I, Saunders TL, and Morrison SJ. Sox17 dependence distinguishes the transcriptional regulation of fetal from adult hematopoietic stem cells. Cell 2007; 130:470-83.
Buffer:
SOX17 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SOX17 antibody was raised against a 16 amino acid peptide near the center of human SOX17.

The immunogen is located within amino acids 240 - 290 of SOX17.
NCBI Gene ID #:
64321
NCBI Official Name:
RY (sex determining region Y)-box 17
NCBI Official Symbol:
SOX17
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa

Observed: 42 kDa
Protein Accession #:
NP_071899
Protein GI Number:
11967991
Purification:
SOX17 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
SOX17 antibody is human, mouse and rat reactive. SOX17 is predicted to not cross-react with other SOX proteins.
Swissprot #:
Q9H6I2
User NOte:
Optimal dilutions for each application to be determined by the researcher.