NOX3 Antibody
| Code | Size | Price |
|---|
| PSI-7925-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7925-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 11136626
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
NOX3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
NAPDH oxidase 3, NOX-3, GP91-3, MOX2, MOX-2
Application Note:
NOX3 antibody can be used for detection of NOX3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith (2,3). It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss (4).
Background References:
- Bedard K and Krause KH. The Nox family of ROS-generating NAPDH oxidases: physiology and pathophysiology. Physiol. Rev. 2007; 87:245-313.
- Cheng G, Cao Z, Xu X, et al. Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene 2001; 269:131-40.
- Paffenholz R, Bergstrom RA, Pasutto F, et al. Vestibular defects in head-tilt mice result from mutations in Nox3, encoding an NADPH oxidase. Genes Dev. 2004; 18:486-91.
- Mukherjea, Jajoo S, Sheehan K, et al. NOX3 NADPH oxidase couples transient receptor potential vanilloid 1 to signal transducer and activator of transcription 1-mediated inflammation and hearing loss. Antioxidants and Redox Signaling 2011; 14:999-1010.
Buffer:
NOX3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
NOX3 antibody was raised against a 15 amino acid peptide near the amino terminus of human NOX3.
The immunogen is located within amino acids 110 - 160 of NOX3.
The immunogen is located within amino acids 110 - 160 of NOX3.
NCBI Gene ID #:
50508
NCBI Official Name:
NADPH oxidase 3
NCBI Official Symbol:
NOX3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 62 kDa
Observed: 72 kDa
Observed: 72 kDa
Protein Accession #:
NP_056533
Protein GI Number:
11136626
Purification:
NOX3 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
NOX3 antibody is human and mouse reactive. NOX3 is predicted to not cross-react with other NOX proteins.
Swissprot #:
Q9HBY0
User NOte:
Optimal dilutions for each application to be determined by the researcher.