VEZT Antibody
| Code | Size | Price |
|---|
| PSI-7959-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7959-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 155030244
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
VEZT antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
VEZT Antibody: VEZATIN, Vezatin
Application Note:
VEZT antibody can be used for detection of VEZT by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
Vezatin (VEZT) is a transmembrane protein which localizes to adherens junctions and binds to myosin VIIA and the cadherin-catenins complex (1). Expression of this gene in gastric cancer tissues is decreased due to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts (2). VEZT has also been shown to be required for morphogenesis of the preimplantation mouse embryo (3).
Background References:
- Kussel-Andermann P, El-Amraoui A, Safieddine S, et al. Vezatin, a novel transmembrane protein, bridges myosin VIIA to the cadherin-catenins complex. EMBO J. 2000; 19:6020-9.
- Guo X, Jing C, Zhang L, et al. Down-regulation of VEZT gene expression in human gastric cancer involves promoter methylation and miR-43c. Biochem. Biophys. Res. Commun. 2011; 404:622-7.
- Hyenne V, Louvet-Vallee S, El-Amraoui A, et al. Vezatin, a protein associated to adherins junctions, is required for mouse blastocyst morphogenesis. Dev. Biol. 2005; 287:180-91.
Buffer:
VEZT antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
VEZT antibody was raised against a 17 amino acid peptide near the center of human VEZT.
The immunogen is located within amino acids 480 - 530 of VEZT.
The immunogen is located within amino acids 480 - 530 of VEZT.
NCBI Gene ID #:
55591
NCBI Official Name:
vezatin, adherens junctions transmembrane protein
NCBI Official Symbol:
VEZT
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 63, 68, 78, 81, 83, 86 kDa
Observed: 60 kDa
Observed: 60 kDa
Protein Accession #:
NP_060069
Protein GI Number:
155030244
Purification:
VEZT antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
VEZT antibody is human, mouse and rat reactive. Multiple isoforms are known to exist.
Swissprot #:
Q9HBM0
User NOte:
Optimal dilutions for each application to be determined by the researcher.