WDR61 Antibody
| Code | Size | Price |
|---|
| PSI-7985-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7985-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 13376840
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
WDR61 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
WD repeat-containing protein 61, Meiotic recombination REC14 protein homolog, REC14, SKI8
Application Note:
WDR61 antibody can be used for detection of WDR61 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
WDR61 (WD-repeat-containing protein 61), also known as SKI8 or REC14, is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (1,2). PAF1C is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C (3,4).
Background References:
- Zhu B, Mandal SS, Pham AD, et al. The human PAF complex coordinates transcription with events downstream of RNA synthesis. Genes Dev. 2005; 19:1668-73.
- Zhu B, Zheng Y, Pham AD, et al. Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation. Mol. Cell. 2005; 20:601-11.
- Kim J, Guermah M, and Roeder RG. The human PAF1 complex acts in chromatin transcription elongation both independently and cooperatively with SII/TFIIS. Cell 2010; 140:491-503.
- Chen Y, Yamaguchi Y, Tsugeno Y, et al. DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation. Genes Dev. 2009; 23):2765-77.
Buffer:
WDR61 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%), Chicken: (79%)
Immunogen:
WDR61 antibody was raised against a 14 amino acid peptide near the amino terminus of human WDR61.
The immunogen is located within the first 50 amino acids of WDR61.
The immunogen is located within the first 50 amino acids of WDR61.
NCBI Gene ID #:
80349
NCBI Official Name:
WD repeat domain 61
NCBI Official Symbol:
WDR61
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 34 kDa
Observed: 30 kDa
Observed: 30 kDa
Protein Accession #:
NP_079510
Protein GI Number:
13376840
Purification:
WDR61 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
WDR61 antibody is human, mouse and rat reactive.
Swissprot #:
Q9GZS3
User NOte:
Optimal dilutions for each application to be determined by the researcher.