PROM1 Antibody

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ProSci
Product Code: PSI-7783
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7783-0.02mg0.02mg£150.00
Quantity:
PSI-7783-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Antibody Clone: 5174387
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PROM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of PROM1 in Jurkat cells with PROM1 antibody at 2.5 μg/mL.
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Immunofluorescence of PROM1 in Jurkat cells with PROM1 antibody at 20 μg/mL.
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Western blot analysis of PROM1 in Jurkat cell lysate with PROM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of PROM1 in Jurkat cells with PROM1 antibody at 2.5 μg/mL.
Immunofluorescence of PROM1 in Jurkat cells with PROM1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PROM1 Antibody: RP41, AC133, CD133, MCDR2, STGD4, CORD12, PROML1, MSTP061, Prominin-1, Antigen AC133
Application Note:
PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).
Background References:
  • Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90:5002-12.
  • Zhang Q, Zulfiqar F, Xiao X, et al. Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene. Hum. Genet. 2007; 122:293-9.
  • Tabu K, Bizen N, Taga T, et al. Gene regulation of Prominin-1 (CD133) in normal and cancerous tissues. Adv. Exp. Med. Biol. 2013; 777:73-85.
Buffer:
PROM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1.

The immunogen is located within the last 50 amino acids of PROM1.
NCBI Gene ID #:
8842
NCBI Official Name:
Prominin 1
NCBI Official Symbol:
PROM1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 95 kDa

Observed: 97 kDa
Protein Accession #:
NP_006008
Protein GI Number:
5174387
Purification:
PROM1 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Cancer
SPECIFICITY:
PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.
Swissprot #:
O43490
User NOte:
Optimal dilutions for each application to be determined by the researcher.