DLK1 (mouse):Fc (human) (rec.)
| Code | Size | Price |
|---|
| AG-40A-0107Y-C010 | 10 ug | £220.00 |
Quantity:
| AG-40A-0107Y-3010 | 3 x 10 ug | £430.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Antibody Clonality: Enzyme
Regulatory Status: RUO
Target Species: Mouse
Shipping:
Blue Ice
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
Protein Delta Homolog 1; Fetal Antigen 1; Preadipocyte Factor 1; Pref-1; FA1
Biological Activity:
Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
Concentration:
0.1mg/ml after reconstitution.
EClass:
32160000
Endotoxin:
<0.1EU/µg purified protein (LAL test; Lonza).
Form (Short):
liquid
Formulation:
Lyophilized. Contains PBS + 0.02% CHAPS.
Handling Advice:
After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1% BSA should be used for further dilutions.
Long Description:
Protein. Signal peptide and extracellular domain of mouse DLK1 (aa 1-305) are fused at the C-terminus to the Fc portion of human IgG1. Source: HEK 293 cells. Endotoxin content: <0.1EU/µg purified protein (LAL test; Lonza). Lyophilized. Contains PBS + 0.02% CHAPS. Purity: >95% (SDS-PAGE). The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-alpha converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Molecular Weight:
~75kDa (SDS-PAGE)
NCBI, Uniprot Number:
Q09163
Package Type:
Plastic Vial
Product Description:
The transmembrane and secreted protein delta-like 1 homolog (DLK1; Protein delta homolog 1; Preadipocyte factor 1; Pref-1) belongs to the EGF-like family. It can be cleaved by a tumor necrosis factor-alpha converting enzyme (TACE) to release a soluble form. DLK1 plays important roles in regulating cell differentiation such as adipogenesis and osteogenesis. Membrane-bound DLK1 promotes hypertrophic myotube formation, whereas soluble DLK1 inhibits myotube formation. The soluble form of DLK1 inhibits preadipocyte differentiation, while membrane DLK1 represses preadipocyte proliferation. DLK1 is also associated with tumor invasion.
Purity:
>95% (SDS-PAGE)
Sequence:
Signal peptide and extracellular domain of mouse DLK1 (aa 1-305) are fused at the C-terminus to the Fc portion of human IgG1.
Source / Host:
HEK 293 cells
TAGs:
Fc
Transportation:
Non-hazardous
UNSPSC Category:
Other Proteins
UNSPSC Number:
12352202
Use & Stability:
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
References
Genes with physiological roles in callipyge muscle hypertrophy: H. Yu; Purde University Diss. Paper 10 (2013) | Preadipocyte factor 1 induces pancreatic ductal cell differentiation into insulin-producing cells: M. Rhee, et al.; Sci. Rep. 6, 23960 (2016) | Identification of genes directly responding to DLK1 signaling in Callipyge sheep: H. Yu, et al.; BMC Genomics 19, 283 (2018) | Preadipocyte factor 1 regulates adipose tissue browning via TNF-alpha-converting enzyme-mediated cleavage: M. Rhee, et al.; Metabolism 101, 153977 (2019)