HES5 Antibody
| Code | Size | Price |
|---|
| PSI-8055-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8055-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
HES5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38
Application Note:
HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
Background References:
- Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
- Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
- Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.
Buffer:
HES5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.
The immunogen is located within the first 50 amino acids of HES5.
The immunogen is located within the first 50 amino acids of HES5.
NCBI Gene ID #:
388585
NCBI Official Name:
hes family bHLH transcription factor
NCBI Official Symbol:
HES5
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 18 kDa
Observed: 17 kDa
Observed: 17 kDa
Protein Accession #:
NP_001010926
Protein GI Number:
58219048
Purification:
HES5 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q5TA89
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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