JAG1 Antibody

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ProSci
Product Code: PSI-8059
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8059-0.02mg0.02mg£150.00
Quantity:
PSI-8059-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
JAG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.
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Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.
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Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.
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Western blot analysis of JAG1 in HeLa cell lysate with JAG1 antibody at 1 μg/ml.
Immunohistochemistry of JAG1 in human spleen tissue with JAG1 antibody at 5 μg/ml.
Immunofluorescence of JAG1 in human spleen tissue with JAG1 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
Jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1
Application Note:
JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1). Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1,2). JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3). JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4,5).
Background References:
  • Lai EC. Notch signaling: control of cell communication and cell fate. Development 2004; 131:965-73.
  • Milner LA, Kopan R, Martin DI, et al. A human homologue of the Drosophila developmental gene, Notch, is expressed in CD34+ hematopoietic precursors. Blood1994; 83:2057-62.
  • Guida V, Chiappe F, Ferese R, et al. Novel and recurrent JAG1 mutations in patients with tetralogy of Fallot. Clin. Genet. 2011; 80:591-4.
  • Santagata S, Demichelis F, Riva A, et al. JAGGED1 expression is associated with prostate cancer metastasis and recurrence. Cancer Res. 2004; 64:6854-7.
Buffer:
JAG1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1.

The immunogen is located within the last 50 amino acids of JAG1.
NCBI Gene ID #:
182
NCBI Official Name:
jagged 1
NCBI Official Symbol:
JAG1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 134 kDa

Observed: 130 kDa
Protein Accession #:
NP_000205
Protein GI Number:
4557679
Purification:
JAG1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle,Stem Cell
SPECIFICITY:
JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.
Swissprot #:
P78504
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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