WNT5 Antibody
| Code | Size | Price |
|---|
| PSI-8063-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8063-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
WNT5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
Wingless-type MMTV integration site family member 5
Application Note:
WNT5 antibody can be used for detection of WNT5 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Background:
WNT5A and WNT5B are near-identical members of the WNT gene family, which consists of structurally related genes which encode secreted signaling proteins that have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis (1,2). WNT5A and WNT5B coordinate chondrocyte proliferation and differentiation by differentially regulating cyclin D1 and p130 expression, as well as chondrocyte-specific Col2a1 expression (3). Up-regulation of WNT5A and WNT5B has been observed in several types of human cancers, suggesting that they may be useful as diagnostic markers or potent therapeutic targets (4).
Background References:
- Saitoh T and Katoh M. Molecular cloning and characterization of human WNT5B on chromosome 12p13.3 region. Int. J. Oncol. 2001; 19:347-51.
- Serio RN. Wnt of the two horizons: putting stem cell self-renewal and cell fate determination into context. Stem Cells Dev. 2014; epub Apr. 24.
- Yang Y, Topol L, Lee H, et al. Wnt5a and Wnt5b exhibit distinct activities in coordinating chondrocyte proliferation and differentiation. Development 2003; 130:1003-15.
- Saitoh T and Katoh M. Expression and regulation of WNT5A and WNT5B in human cancer: up-regulation of WNT5A by TNFalpha in MKN45 cells and up-regulation of WNT5B by beta-estradiol in MCF-7 cells. Int. J. Mol. Med. 2002; 10:345-9.
Buffer:
WNT5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
Immunogen:
WNT5 antibody was raised against a 15 amino acid peptide near the center of human WNT5B.
The immunogen is located within amino acids 130 - 180 of WNT5.
The immunogen is located within amino acids 130 - 180 of WNT5.
NCBI Gene ID #:
81029
NCBI Official Name:
Wnt family member 5B
NCBI Official Symbol:
WNT5B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa
Observed: 465 kDa
Observed: 465 kDa
Protein Accession #:
NP_110402
Protein GI Number:
17402919
Purification:
WNT5 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle,Stem Cell
SPECIFICITY:
WNT5 antibody is human, mouse and rat reactive. WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q9H1J7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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