JAK2 Antibody

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ProSci
Product Code: PSI-8085
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8085-0.02mg0.02mg£150.00
Quantity:
PSI-8085-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
JAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.
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Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.
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Western blot analysis of JAK2 in HeLa cell lysate with JAK2 antibody at 1 μg/ml.
Immunohistochemistry of JAK2 in mouse kidney tissue with JAK2 antibody at 5 μg/ml.

Documents

Further Information

Additional Names:
Janus kinase 2, JTK10, THCYT3
Application Note:
JAK2 antibody can be used for detection of JAK2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
JAK2 is a member of the JAK family of kinases that also include JAK1, JAK3, and TYK2 (1,2). JAK kinases are activated following tyrosine phosphorylation of cytokine receptors after ligand binding. JAK2 activation promotes the recruitment and phosphorylation of STAT3 and STAT5. These transcription factors then translocate to the nucleus where they bind specific DNA promoter sequences resulting in the transcription of genes that regulate cell proliferation, differentiation, and apoptosis (3). Mice with a disrupted JAK2 gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis (4).
Background References:
  • Wilks AF, Harpur AG, Kurban RR, et al. Two novel protein-tyrosine kinases, each with a second phosphotransferase-related catalytic domain, define a new class of protein kinase. Mol. Cell Biol. 1991; 11:2057-66.
  • Quintas-Cardama A and Verstovsek S. Molecular pathways: JAK/STAT pathway: mutations, inhibitors, and resistance. Clin. Can. Res. 2013; 19:1933-40.
  • Levine RL, Pardanani A, Tefferi A, et al. Role of JAK2 in the pathogenesis and therapy of myeloproliferative disorders. Nat. Rev. Cancer 2007; 7:673-83.
  • Neubauer H, Cumano A, Muller M, et al. Jak2 deficiency defines an essential developmental checkpoint in definitive hematopoiesis. Cell 1998; 93:397-409.
Buffer:
JAK2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (82%)
Immunogen:
JAK2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human JAK2.

The immunogen is located within the last 50 amino acids of JAK2.
NCBI Gene ID #:
3717
NCBI Official Name:
Janus kinase 2
NCBI Official Symbol:
JAK2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 125 kDa

Observed: 125 kDa
Protein Accession #:
NP_004963
Protein GI Number:
4826776
Purification:
JAK2 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Apoptosis
SPECIFICITY:
JAK2 antibody is human, mouse and rat reactive. JAK2 antibody is predicted to not cross-react with other members of the JAK family of proteins.
Swissprot #:
O60674
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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