PRRX1 Antibody

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ProSci
Product Code: PSI-8093
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8093-0.02mg0.02mg£150.00
Quantity:
PSI-8093-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
PRRX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Images

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Western blot analysis of PRRX1 in HeLa cell lysate with PRRX1 antibody at 1 μg/ml.
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Western blot analysis of PRRX1 in HeLa cell lysate with PRRX1 antibody at 1 μg/ml.

Documents

Further Information

Additional Names:
Paired related homeobox 1, homeobox protein PHOX1, Paired mesoderm homeobox protein 1, PHOX1, PMX1, PRX1, PRX-1
Application Note:
PRRX1 antibody can be used for detection of PRRX1 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
The paired related homeobox protein 1 (PRRX1) is a paired-type DNA-binding homeodomain that plays an important role as a key switch for neural cell lineage determination and is essential for development (1,2). PRRX1 is expressed in a number of undifferentiated mesenchymal cells in mouse embryos and adults (3). Mice with a disrupted PRRX1 gene show perinatal death with craniofacial and limb malformations (4). Low PRRX1 expression levels were significantly associated with metastasis and poor prognosis of breast and colorectal cancer (5).
Background References:
  • Nohno T, Koyama E, Myokai F, et al. Chicken homeobox gene related to Drosophila paired is predominantly expressed in the developing limb. Dev. Biol. 1993; 158:254?64.
  • Shimozaki K, Clemenson GD Jr, and Gage FH. Paired related homeobox protein 1 is a regulator of stemness in adult neural stem/progenitor cells. J. Neurosci. 2013; 33:4066-75.
  • Cserjesi P, Lilly B, Bryson L, et al. MHox: a mesodermally restricted homeodomain protein that binds an essential site in the muscle creatine kinase enhancer. Development 1992; 115:1087?101.
  • Lu MF, Cheng HT, Kern MJ, et al. prx-1 functions cooperatively with another paired-related homeobox gene, prx-2, to maintain cell fates within the craniofacial mesenchyme. Development 1999; 126:495?504.
Buffer:
PRRX1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
PRRX1 antibody was raised against a 19 amino acid peptide near the center of human PRRX1.

The immunogen is located within amino acids 70 - 120 of PRRX1.
NCBI Gene ID #:
5396
NCBI Official Name:
paired related homeobox 1
NCBI Official Symbol:
PRRX1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 27 kDa

Observed: 26 kDa
Protein Accession #:
NP_073207
Protein GI Number:
12707577
Purification:
PRRX1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle,Stem Cell
SPECIFICITY:
PRRX1 antibody is human, mouse and rat reactive. At least two isoforms of PRRX1 are known to exist; this antibody will detect both isoforms.
Swissprot #:
P54821
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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