Vinculin Antibody [7E10]
| Code | Size | Price |
|---|
| PSI-PM-7811-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-PM-7811-0.1mg | 0.1mg | £515.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Vinculin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Documents
Further Information
Additional Names:
CMD1W, CMH15, Metavinculin, MVCL
Application Note:
Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background References:
- Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
- Gilmore et al. J. Cell Sci. 1992; 103:719-31.
- Deakin et al. PLoS One 2012; 7:e37990.
- Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Buffer:
Vinculin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen:
Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.
The immunogen is located within amino acids 50 - 100 of Vinculin.
The immunogen is located within amino acids 50 - 100 of Vinculin.
NCBI Gene ID #:
7414
NCBI Official Name:
vinculin
NCBI Official Symbol:
VCL
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 117 kDa
Observed: 120 kDa
Observed: 120 kDa
Protein Accession #:
NP_003364
Protein GI Number:
4507877
Purification:
Vinculin antibody is purified via Protein A prification.
Research Area:
Homeostasis
SPECIFICITY:
Vinculin antibody is human, mouse, rat, rabbit and chicken reactive. At least three isoforms of Vinculin are known to exist.
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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