Pan Cytokeratin monoclonal antibody, clone AE1 + AE3
| Code | Size | Price |
|---|
| MAB11311-100ug | 100 ug | £488.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG1, kappa
Antibody Clonality: Monoclonal
Antibody Clone: AE1 + AE3
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Store at 4 °C. For long term storage store at -20 °C. Aliquot to avoid repeated freezing and thawing.
Documents
Further Information
Antigen Target Species:
Human
Form:
Liquid
Gene Description:
keratin 77
Gene ID:
374454|51350
Gene Name:
KRT77
Immunogen:
Human epidermal keratin.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product Description:
Mouse monoclonal antibody raised against Pan Cytokeratin.
Recommend Dilutions:
Flow Cytometry (1-2 ug/million cells)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.25-0.5 ug/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM
Specificity:
This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19). AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Storage Buffer:
In PBS (0.05% BSA, 0.05% sodium azide).