5-DTAF hydrochloride
| Code | Size | Price |
|---|
| CDX-D0123-M500 | 500 mg | £194.00 |
Quantity:
| CDX-D0123-GG25 | 2.5 g | £743.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
AMBIENT
Storage:
-20°C
Documents
Further Information
Alternate Names/Synonyms:
5-[(4,6-Dichlorotriazin-2-yl)amino]fluorescein hydrochloride; NSC378139
Appearance:
Yellow to orange powder.
CAS:
21811-74-5
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H
InChiKey:
CEKSHCAIHGNWBK-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 21811-74-5. Formula: C23H12Cl2N4O5 . HCl. MW: 495.3 . 36.5. Synthetic. 5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
MDL:
MFCD00051407
Molecular Formula:
C23H12Cl2N4O5 . HCl
Molecular Weight:
495.3 . 36.5
Package Type:
Vial
Product Description:
5-DTAF is highly reactive with proteins. Unlike other reactive fluoresceins, 5-DTAF not only reacts with amino groups, but also reacts with thiol groups and even directly reacts with hydroxy groups such as polysaccharides and other alcohols in aqueous solution at pH above 9. Due to its high reactivity, 5-DTAF is also used to label carbohydrates. Spectral properties: Abs/Em = 492/517 nm
Purity:
>98% (HPLC)
SMILES:
Cl.OC(=O)C1=C(C=CC(NC2=NC(Cl)=NC(Cl)=N2)=C1)C1=C2C=CC(=O)C=C2OC2=CC(O)=CC=C12
Solubility Chemicals:
Soluble in chloroform.
Source / Host:
Synthetic.
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at -20°C.
References
(1) D. Blakeslee & M.G. Baines; J. Immunol. Methods 13, 305 (1976) | (2) D. Blakeslee; J. Immunol. Methods 17, 361 (1977) | (3) R. Siegler, et al.; J .Pharm. Biomed. Anal. 7, 45 (1989) | (4) S. Prigent-Richard, et al.; J. Biomed. Mater. Res. 40, 275 (1998) | (5) K.N. Krahn, et al.; Anal. Biochem. 350, 177 (2006) | (6) M. Ahmed, et al.; J. Ind. Microbiol. Biotechnol. 38, 249 (2011) | (7) S.E. Szczesny, et al.; PLoS One 9, e99588 (2014)