TMB dihydrochloride
| Code | Size | Price |
|---|
| CDX-T0151-G001 | 1 g | £65.00 |
Quantity:
| CDX-T0151-G005 | 5 g | £230.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Regulatory Status: RUO
Shipping:
Ambient
Storage:
+4°C
Documents
Further Information
Alternate Names/Synonyms:
3,3',5,5'-Tetramethylbenzidine dihydrochloride; TMBZ
Appearance:
White to off-white to pink powder.
CAS:
64285-73-0
EClass:
32160000
Form (Short):
liquid
Handling Advice:
Keep cool and dry.Protect from light and moisture.
InChi:
InChI=1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
InChiKey:
NYNRGZULARUZCC-UHFFFAOYSA-N
Long Description:
Chemical. CAS: 64285-73-0. Formula: C16H20N2 . 2HCl. MW: 240.3 . 72.9. Synthetic TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
MDL:
MFCD00012961
Molecular Formula:
C16H20N2 . 2HCl
Molecular Weight:
240.3 . 72.9
Package Type:
Vial
Product Description:
TMBZ is a chromogenic reagent utilized for peroxidase detection. It has been developed as an alternative to benzidine. Although the TMBZ solution is colorless, it turns bluish-green (lambdamax: 655 nm) in the presence of hydrogen peroxide and peroxidase. The structure of this bluish-green complex is thought to be a radical form of two oxidized TMBZ molecules. This peroxidase substrate is used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2M H2SO4 (resulting in a yellow color), and read at 450nm.
Purity:
>98% (Titration)
SMILES:
Cl[H].Cl[H].CC1=CC(=CC(C)=C1N)C1=CC(C)=C(N)C(C)=C1
Solubility Chemicals:
Soluble in DMSO.
Source / Host:
Synthetic
Transportation:
Non-hazardous
UNSPSC Category:
Fluorescent Reagents
UNSPSC Number:
41105331
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
References
(1) V.R. Holland, et al.; Tetrahedron 30, 3299 (1974) | (2) P.E. Thomas, et al.; Anal. Biochem. 75, 168 (1976) | (3) H.H. Liem, et al.; Anal. Biochem. 98, 388 (1979) | (4) E.S. Bos, et al.; J. Immunoassay 2, 187 (1981) | (5) K. Suzuki, et al.; Anal. Biochem. 132, 345 (1983) | (6) F.H. Pujol, et al.; J. Immunoassay. 14, 21 (1993) | (7) F.B. Serrat; Talanta 41, 2091 (1994)