beta-Actin Antibody [10B7]
| Code | Size | Price |
|---|
| PSI-PM-7547-biotin-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-PM-7547-biotin-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 10B7
Regulatory Status: RUO
Target Species:
- Chicken (Gallus)
- Drosophila (fruit fly)
- Human
- Mouse
- Puumala Virus
- Rat
- Zebrafish
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Biotin-Actin, Beta-Actin, Actin
Application Note:
beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 μ/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
- Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
- Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.
Buffer:
beta-Actin antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of beta-actin.
NCBI Gene ID #:
60
NCBI Official Name:
actin, beta
NCBI Official Symbol:
ACTB
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42 kDa
Observed: 42 kDa
Observed: 42 kDa
Protein Accession #:
AAH02409
Protein GI Number:
12803203
Purification:
beta-Actin Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Swissprot #:
P60709
User NOte:
Optimal dilutions for each application to be determined by the researcher.