DDDDK Antibody [1D1B12]

ProSci
Product Code: PSI-PM-7657-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7657-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7657-biotin-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 1D1B12
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Biotin-DDDDK antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of (1) 1000ng, (2) 500ng, (3) 250ng, and (4) 125ng of DDDDK-tagged recombinant protein (GGP1) with Biotin-DDDDK-tag antibody at 0.5 μg/ml.

Western blot analysis of (1) 1000ng, (2) 500ng, (3) 250ng, and (4) 125ng of DDDDK-tagged recombinant protein (GGP1) with Biotin-DDDDK-tag antibody at 0.5 μg/ml.

Further Information

Additional Names:
Biotin-DDDDK, DDDDK, FLAG, FLAGtag,flag-tag
Application Note:
Biotin-DDDDK antibody can be used for detection of DDDDK by Western blot at 0.5 - 1 μ/ml.
Background:
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. DDDDK-tag is one of the more popular epitope tags for expressed recombinant proteins.
Buffer:
Biotin-DDDDK antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Biotin-DDDDK antibody was raised against a synthetic peptide containing DYKDDDDK.
NCBI Gene ID #:
60
NCBI Official Name:
actin, beta
NCBI Official Symbol:
ACTB
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
N/A
Purification:
Biotin-DDDDK antibody is affinity chromatography purified via peptide column.
SPECIFICITY:
Biotin-DDDDK-tag antibody will detect carboxy-terminal DDDDK-tagged recombinant proteins.
Swissprot #:
P60709
User NOte:
Optimal dilutions for each application to be determined by the researcher.