SLC1A7 Antibody
| Code | Size | Price |
|---|
| PSI-8083-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8083-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
SLC1A7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Solute carrier family 1 (glutamate transporter) member 7, AAAT, excitatory amino acid transporter 5, EAAT5
Application Note:
SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
Background References:
- Arriza JL, Eliasof S, Kavanaugh MP, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 1997; 94:4155-60.
- Lee A, Anderson AR, Stevens M, et al. Excitatory amino acid transporter 5 is widely expressed in peripheral tissues. Eur. J. Histochem. 2013; 57:e11.
- Schneider N, Cordeiro S, Machtens JP, et al. Functional properties of the retinal glutamate transporters GLT-1c and EAAT5. J. Biol. Chem. 2014; 289:1815-24.
- Boehmer C, Rajamanickam J, Schniepp R, et al. Regulation of the excitatory amino acid transporter EAAT5 by the serum and glucocorticoid dependent kinases SGK1 and SGK3. Biochem. Biophys. Res. Commun. 2005; 329:738-42.
Buffer:
SLC1A7 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SLC1A7 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLC1A7.
The immunogen is located within amino acids 460 - 510 of SLC1A7.
The immunogen is located within amino acids 460 - 510 of SLC1A7.
NCBI Gene ID #:
6512
NCBI Official Name:
solute carrier family 1 (glutamate transporter), member 7
NCBI Official Symbol:
SLC1A7
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 17, 52, 62, 68 kDa
Observed: 52 kDa
Observed: 52 kDa
Protein Accession #:
NP_001274524
Protein GI Number:
567316146
Purification:
SLC1A7 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.
Swissprot #:
O00341
User NOte:
Optimal dilutions for each application to be determined by the researcher.