HAT-tag Antibody [4A6F3]

ProSci
Product Code: PSI-PM-7661-biotin
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-7661-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-PM-7661-biotin-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: 12D3D5
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
HAT-tag antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

1 / 1
Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml.

Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml.

Further Information

Additional Names:
Hat-tag, Hat
Application Note:
HAT-tag antibody can be used for detection of HAT-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.
Background:
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HAT-tag is one of the more popular epitope tags for expressed recombinant proteins.
Buffer:
HAT-tag antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.
NCBI Official Name:
N/A
NCBI Official Symbol:
N/A
NCBI Organism:
N/A
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
N/A
Purification:
HAT-tag antibody is affinity chromatography purified via peptide column.
Research Area:
Other
SPECIFICITY:
HAT-tag antibody will detect amino- or carboxy-terminal HAT-tagged recombinant proteins.
Swissprot #:
N/A
User NOte:
Optimal dilutions for each application to be determined by the researcher.