IKZF2 Antibody
| Code | Size | Price |
|---|
| PSI-8117-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8117-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
IKZF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Ikaros family zinc finger 2 (Helios), ANF1A2, HELIOS, ZNF1A2, ZNFN1A2
Application Note:
IKZF2 antibody can be used for detection of IKZF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Ikaros family zinc finger 2 (IKZF2) is a member of the Ikaros family of zinc-finger proteins, a family of hematopoietic-specific transcription factors involved in the regulation of lymphocyte development (1,2). IKZF2 forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development (3). Its expression is a marker for T cell activation and proliferation (4).
Background References:
- Hahm K, Cobb BS, McCarty AS, et al. Helios, a T cell-restricted Ikaros family member that quantitatively associates with Ikaros at centromeric heterochromatin. Genes Dev. 1998; 12:782-96.
- John LB and Ward AC. The Ikaros gene family: transcriptional regulators of hematopoiesis and immunity. Mol. Immunol. 2011; 48:1272-8.
- Kelly CM, Ikeda T, Koipally J, et al. Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 1998; 8:508-15.
- Akimova T, Beier UH, Wang L, et al. Helios expression is a marker for T cell activation and proliferation. PLoS One 2011; 6:e24226.
Buffer:
IKZF2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IKZF2 antibody was raised against a 19 amino acid peptide near the amino terminus of human IKZF2.
The immunogen is located within amino acids 50 - 100 of IKZF2.
The immunogen is located within amino acids 50 - 100 of IKZF2.
NCBI Gene ID #:
22807
NCBI Official Name:
Ikaros family zinc finger 2 (Helios)
NCBI Official Symbol:
IKZF2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58 kDa
Observed: 60 kDa
Observed: 60 kDa
Protein Accession #:
NP_057344
Protein GI Number:
119220596
Purification:
IKZF2 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Immunology
SPECIFICITY:
IKZF2 antibody is human, mouse and rat reactive. At least two isoforms of IKZF2 are known to exist; this antibody will detect both isoforms. IKZF2 antibody is predicted to not cross-react with other IKZF proteins.
Swissprot #:
Q9UKS7
User NOte:
Optimal dilutions for each application to be determined by the researcher.