SOX4 Antibody
| Code | Size | Price |
|---|
| PSI-8123-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8123-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
SOX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
SRY (Sex determining region Y)-box 4, Transcription factor SOX-4, EVI16
Application Note:
SOX4 antibody can be used for detection of SOX4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate (1,2). It may act as a transcriptional regulator after forming a protein complex with other proteins, such as syndecan binding protein (syntenin) (3). SOX4 contains one HMG box DNA-binding domain and may function in the apoptosis pathway leading to cell death. It has also been suggested to play a role in tumorigenesis and may mediate downstream effects of parathyroid hormone (PTH) and PTH-related protein (PTHrP) in bone development (4,5).
Background References:
- Wotton D, Lake RA, Farr CJ, et al. The high mobility group transcription factor, SOX4, transactivates the human CD2 enhancer. J. Biol. Chem. 1995; 270:7515-22.
- Pan X, Li H, Zhang P, et al. Ubc9 interacts with SOX4 and represses its transcriptionalactivity. Biochem. Biophys. Res. Commun. 2006; 344:727-34.
- Castillo SD, Matheu A, Mariani N, et al. Novel transcriptional targets of the SRY-HMG box transcription factor SOX4 link its expression to the development of small cell lung cancer. Cancer Res. 2012; 72:176-86.
- Zhang H, Alberich-Jorda M, Amabile G, et al. Sox4 is a key oncogenic target in C/EBPa mutant acute myeloid leukemia. Cancer Cell 2013; 24:575-88.
Buffer:
SOX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SOX4 antibody was raised against a 16 amino acid peptide near the amino terminus of human SOX4.
The immunogen is located within the first 50 amino acids of SOX4.
The immunogen is located within the first 50 amino acids of SOX4.
NCBI Gene ID #:
6659
NCBI Official Name:
SRY (Sex determining region Y)-box 4
NCBI Official Symbol:
SOX4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52 kDa
Observed: 520 kDa
Observed: 520 kDa
Protein Accession #:
NP_003098
Protein GI Number:
4507163
Purification:
SOX4 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Apoptosis
SPECIFICITY:
SOX4 antibody is human, mouse and rat reactive.
Swissprot #:
Q06945
User NOte:
Optimal dilutions for each application to be determined by the researcher.