ARID1A Antibody
| Code | Size | Price |
|---|
| PSI-8173-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8173-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
ARID1A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1
Application Note:
ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
Background References:
- Martens JA and Winston F. Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr. Opin. Genet. Dev. 2003; 13:136-42.
- Nie Z, Xue Y, Yang D, et al. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Mol. Cell. Biol. 2000; 20:8879-88.
Buffer:
ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.
The immunogen is located within amino acids 580 - 630 of ARID1A.
The immunogen is located within amino acids 580 - 630 of ARID1A.
NCBI Gene ID #:
8289
NCBI Official Name:
AT rich interactive domain 1A (SWI-like)
NCBI Official Symbol:
ARID1A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 133, 227, 251 kDa
Observed: 130, 260 kDa
Observed: 130, 260 kDa
Protein Accession #:
NP_006006
Protein GI Number:
21264565
Purification:
ARID1A antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Signal Transduction
SPECIFICITY:
ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
Swissprot #:
O14497
User NOte:
Optimal dilutions for each application to be determined by the researcher.