GAPDH [12D3H9] Antibody
| Code | Size | Price |
|---|
| PSI-PM-7571-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-PM-7571-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Application Note:
GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Background:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer?s and Huntington?s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Background References:
- Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
- Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
Buffer:
GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%), Sheep: (93%), Bovine: (93%), Chicken: (93%), Rabbit: (93%)
Immunogen:
GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
The immunogen is located within amino acids 230 - 280 of GAPDH.
The immunogen is located within amino acids 230 - 280 of GAPDH.
NCBI Gene ID #:
2597
NCBI Official Name:
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 37 kDa
Observed: 36 kDa
Observed: 36 kDa
Protein Accession #:
NP_002037
Protein GI Number:
7669492
Purification:
GAPDH antibody is Protein A purified.
Research Area:
Signal Transduction,Neuroscience,Homeostasis
SPECIFICITY:
GAPDH antibody is human, mouse,and rat reactive.
Swissprot #:
P04406
User NOte:
Optimal dilutions for each application to be determined by the researcher.