DIRAS3 Antibody
| Code | Size | Price |
|---|
| PSI-8139-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8139-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
DIRAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2
Application Note:
DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).
Background References:
- Yu Y, Xu F, Peng H, et al. NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas. Proc. Natl. Acad. Sci. USA 1999; 96:214-9.
- Lu Z, Luo RZ, Lu Y, et al. The tumor suppressor gene ARHI regulates autophagy and tumor dormancy in human ovarian cancer cells. J. Clin. Invest. 2008; 118:3917-29.
- Klingauf M, Beck M, Berge U, et al. The tumour suppressor DiRas3 interacts with C-RAF and downregulates MEK activity to restrict cell migration. Biol. Cell 2013; 105:91-107.
Buffer:
DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3.
The immunogen is located within the last 50 amino acids of DIRAS3.
The immunogen is located within the last 50 amino acids of DIRAS3.
NCBI Gene ID #:
9077
NCBI Official Name:
DIRAS family, GTP-binding RAS-like 3
NCBI Official Symbol:
DIRAS3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 25 kDa
Observed: 24 kDa
Observed: 24 kDa
Protein Accession #:
NP_004666
Protein GI Number:
4757772
Purification:
DIRAS3 antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Cancer,Autophagy
SPECIFICITY:
DIRAS3 antibody is human specific.
Swissprot #:
O95661
User NOte:
Optimal dilutions for each application to be determined by the researcher.