UBD Antibody
| Code | Size | Price |
|---|
| PSI-8185-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8185-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
UBD antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Ubiquitin D, Diubiquitin, FAT10, GABBR1, UBD-3, Ubiquitin-like protein FAT10
Application Note:
UBD antibody can be used for detection of UBD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
UBD, also designated ubiquitin D or diubiquitin, plays a role in antigen presentation, cytokine response and apoptosis. It is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin (1,2). UBD is inducible by interferon gamma and TNF-alpha and regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (3). It may also regulate cell growth during dendritic cell or B cell activation and development (4,5).
Background References:
- Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells. Lab. Invest. 2006; 86:724-37.
- Cook WJ, Jeffrey LC, Carson M, et al. Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2). J. Biol. Chem. 1992; 267:16467-71.
- Rahighi S, Ikeda F, Kawasaki M, et al. Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation. Cell 2009;136:1098-109.
- Cort L, Habib M, Eberwine RA, et al. Diubiquitin (Ubd) is a susceptibility gene for virus-triggered autoimmune diabetes in rats. Genes Immun. 2014; 15:168-75.
Buffer:
UBD antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%)
Immunogen:
UBD antibody was raised against a 16 amino acid peptide near the center of human UBD.
The immunogen is located within amino acids 80 - 130 of UBD.
The immunogen is located within amino acids 80 - 130 of UBD.
NCBI Gene ID #:
10537
NCBI Official Name:
Ubiquitin D
NCBI Official Symbol:
UBD
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 18 kDa
Observed: 17 kDa
Observed: 17 kDa
Protein Accession #:
NP_006389
Protein GI Number:
222352096
Purification:
UBD antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis,Immunology
SPECIFICITY:
UBD antibody is human specific.
Swissprot #:
O15205
User NOte:
Optimal dilutions for each application to be determined by the researcher.