HOPX Antibody
| Code | Size | Price |
|---|
| PSI-8143-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8143-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
HOPX antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
HOP homeobox, homeodomain-only protein, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO
Application Note:
HOPX antibody can be used for detection of HOPX by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
HOPX is a small homeodomain protein that lacks normally conserved residues required for DNA binding (1). It is thought to act downstream of NKX2-5 and modulates serum response factor (SRF)-dependent cardiac-specific gene expression and cardiac development (1). HOPX also acts a tumor suppressor gene in multiple tumors and cancer cell lines (2,3). HOPX has been reported to regulate the proliferation/differentiation homeostasis in different cell types, including keratinocytes (4).
Background References:
- Chen F, Kook H, Milewski R, et al. Hop is an unusual homeobox gene that modulates cardiac development. Cell 2002; 110:713-23.
- Asanoma K, Matsuda T, Kondo H, et al. NECC1, a candidate choriocarcinoma suppressor gene that encodes a homeodomain consensus motif. Genomics 2003; 81:15-25.
- Katoh H, Yamashita K, Waraya M, et al. Epigenetic silencing of HOPX promotes cancer progression in colorectal cancer. Neoplasia 2012; 14:559-71.
- Obarzanek-Fojt M, Favre B, Kypriotou M, et al. Homeodomain-only protein HOP is a novel modulator of late differentiation in keratinocytes. Eur. J. Cell Biol. 2011; 90:279-90.
Buffer:
HOPX antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (88%), Mouse: (88%), Bovine: (88%), Pig: (81%), Chicken: (81%)
Immunogen:
HOPX antibody was raised against a 16 amino acid peptide near the amino terminus of human HOPX.
The immunogen is located within the first 50 amino acids of HOPX.
The immunogen is located within the first 50 amino acids of HOPX.
NCBI Gene ID #:
84525
NCBI Official Name:
HOP homeobox
NCBI Official Symbol:
HOPX
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 12 kDa
Observed: 18 kDa
Observed: 18 kDa
Protein Accession #:
NP_001138932
Protein GI Number:
224451025
Purification:
HOPX antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Stem Cell
SPECIFICITY:
HOPX antibody is human specific. At least three isoforms of HOPX are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q9BPY8
User NOte:
Optimal dilutions for each application to be determined by the researcher.