LIMA1 Antibody
| Code | Size | Price |
|---|
| PSI-8053-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8053-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
LIMA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
LIM domain and actin-binding protein 1, PP624, EPLIN, SREBP3
Application Note:
LIMA1 antibody can be used for detection of LIMA1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
Background References:
- Maul RS and Chang DD. EPLIN, epithelial protein lost in neoplasm. Oncogene 1999; 18:7838-41.
- Maul RS, Song Y, Amann KJ, et al. EPLIN regulates actin dynamics by cross-linking and stabilizing filaments. J. Cell Biol. 2003; 160:399-407.
- Abe K and Takeichi M. EPLIN mediates linkage of the cadherin catenin complex to F-actin and stabilizes the circumferential actin belt. Proc. Natl. Acad. Sci. USA 2008; 105:13-9.
- Zhang S, Wang X, Iqval S, et al. Epidermal growth factor promotes protein degradation of epithelial protein lost in neoplasm (EPLIN), a putative metastasis suppressor, during epithelial-mesenchymal transition. J. Biol. Chem. 2013; 288:1469-79.
Buffer:
LIMA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%), Pig: (72%)
Immunogen:
LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1.
The immunogen is located within amino acids 230 - 280 of LIMA1.
The immunogen is located within amino acids 230 - 280 of LIMA1.
NCBI Gene ID #:
51474
NCBI Official Name:
LIM domain and actin binding 1
NCBI Official Symbol:
LIMA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50, 66, 84 kDa
Observed: 65 kDa
Observed: 65 kDa
Protein Accession #:
NP_001107018
Protein GI Number:
165905589
Purification:
LIMA1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Homeostasis
SPECIFICITY:
LIMA1 antibody is human specific. At least four isoforms of LIMA1 are known to exist; this antibody will only detect the three largest isoforms.
Swissprot #:
Q9UHB6
User NOte:
Optimal dilutions for each application to be determined by the researcher.