WSB1 Antibody
| Code | Size | Price |
|---|
| PSI-8161-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8161-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
WSB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
WD repeat and SOCS box containing 1, SOCS box-containing WD protein SWiP-1, SWIP1, WD repeat and SOCS box-containing protein 1, WSB-1
Application Note:
WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).
Background References:
- Vasiliauskas D, Hancock S, Stern CD. SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development. Mech. Dev. 1999; 82:79-94.
- Nara H, Onoda T, Rahman M, et al. WSB-1, a novel IL-21 receptor binding molecule, enhances the maturation of IL-21 receptor. Cell Immunol. 2011; 269:54-9.
- Lv W, Zhang Y, Wu Z, et al. Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition. Acta Biochim. Biophys. Sin (Shanghai) 2008; 40:478-88.
- Archange C, Nowak J, Garcia S, et al. The WSB1 gene is involved in pancreatic cancer progression. PLoS One 2008; 3:e2475.
Buffer:
WSB1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (82%)
Immunogen:
WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1.
The immunogen is located within amino acids 70 - 120 of WSB1.
The immunogen is located within amino acids 70 - 120 of WSB1.
NCBI Gene ID #:
26118
NCBI Official Name:
WD repeat and SOCS box containing 1
NCBI Official Symbol:
WSB1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa
Observed: 46 kDa
Observed: 46 kDa
Protein Accession #:
NP_056441
Protein GI Number:
18677720
Purification:
WSB1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Cancer
SPECIFICITY:
WSB1 antibody is human specific.
Swissprot #:
Q9Y6I7
User NOte:
Optimal dilutions for each application to be determined by the researcher.