QSOX1 Antibody

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ProSci
Product Code: PSI-8171
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8171-0.02mg0.02mg£150.00
Quantity:
PSI-8171-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
QSOX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of QSOX1 in EL4 cell lysate with QSOX1 antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of QSOX1 in human spleen tissue with QSOX1 antibody at 5 μg/mL.
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Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.
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Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.
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Western blot analysis of QSOX1 in EL4 cell lysate with QSOX1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of QSOX1 in human spleen tissue with QSOX1 antibody at 5 μg/mL.
Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.
Immunofluorescence of QSOX1 in human spleen tissue with QSOX1 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
Quiescin Q6 sulfhydryl oxidase 1, Sulfhydryl oxidase 1, Q6, QSCN6
Application Note:
QSOX1 antibody can be used for detection of QSOX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The QSOX1 gene, also known as Quiescin Q6, is a fusion of two ancient genes: thioredoxin and ERV1. Its expression is induced as fibroblasts begin to exit the proliferative cycle and enter quiescence, suggesting that this gene plays an important role in growth regulation (1). The QSOX1 protein oxidizes sulfhydryl groups to form disulfide bonds in proteins. QSOX1 expression is induced by hypoxia (2) and appears to protect cells against oxidative stress-induced apoptosis (3).
Background References:
  • Coppock DL, Cina-Poppe D, and Gilleran S. The quiescin Q6 gene (QSCN6) is a fusion of two ancient gene families: thioredoxin and ERV1. Genomics 1998; 54:460-8.
  • Shi CY, Fan Y, Liu B, et al. HIF1 contributes to hypoxia-induced pancreatic cancer cells invasion via promoting QSOX1 expression. Cell Physiol. Biochem. 2013; 32:561-8.
  • Morel C, Adami P, Musard JF, et al. Involvement of sulfhydryl oxidase QSOX1 in the protection of cells against oxidative stress-induced apoptosis. Exp. Cell Res. 2007; 313:3971-82.
Buffer:
QSOX1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (76%)
Immunogen:
QSOX1 antibody was raised against a 17 amino acid peptide near the center of human QSOX1.

The immunogen is located within amino acids 360 - 410 of QSOX1.
NCBI Gene ID #:
5768
NCBI Official Name:
quiescin Q6 sulfhydryl oxidase 1
NCBI Official Symbol:
QSOX1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 65, 82 kDa

Observed: 66 kDa
Protein Accession #:
NP_002817
Protein GI Number:
13325075
Purification:
QSOX1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cell Cycle,Cancer,Apoptosis
SPECIFICITY:
QSOX1 antibody is human and mouse reactive. QSOX1 antibody is predicted to not cross-react with QSOX2.
Swissprot #:
O00391
User NOte:
Optimal dilutions for each application to be determined by the researcher.