RSPO3 Antibody

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ProSci
Product Code: PSI-8153
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8153-0.02mg0.02mg£150.00
Quantity:
PSI-8153-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
RSPO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.
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Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.
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Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.
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Western blot analysis of RSPO3 in 293 cell lysate with RSPO3 antibody at 1 μg/ml.
Immunocytochemistry of RSPO3 in 293 cells with RSPO3 antibody at 5 μg/mL.
Immunofluorescence of RSPO3 in 293 cells with RSPO3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
R-spondin-3, CRISTIN1, PWTSR, THSD2
Application Note:
RSPO3 antibody can be used for detection of RSPO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RSPO3 is a member of the R-spondin family and plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis (1,2). RSPO3 has been shown to be involved in regulating the balance between angioblast and blood cell specification during embryonic vasculogenesis and angiogenesis and may be involved in tumor development (3,4).
Background References:
  • Chen JZ, Wang S, Tang R, et al. Cloning and identification of a cDNA that encodes a novel human protein with thrombospondin type I repeat domain, hPWTSR. Mol. Biol. Rep. 2002; 29:287-92.
  • Kim KA, Wagle M, Tran K, et al. R-Spondin family members regulate the Wnt pathway by a common mechanism. Mol. Biol. Cell 2008; 19:2588-96.
  • Kazanskaya O, Ohkawara B, Heroult M, et al. The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development. Development 2008; 3655-64.
  • Seshagiri S, Stawiski EW, Durinck S, et al. Recurrent R-spondin fusions in colon cancer. Nature 2012; 488:660-4.
Buffer:
RSPO3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RSPO3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RSPO3.

The immunogen is located within amino acids 200 - 250 of RSPO3.
NCBI Gene ID #:
84870
NCBI Official Name:
R-spondin 3
NCBI Official Symbol:
RSPO3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 30 kDa

Observed: 30 kDa
Protein Accession #:
NP_116173
Protein GI Number:
18490982
Purification:
RSPO3 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
RSPO3 antibody is human and mouse reactive. At least three isoforms of RSPO3 are known to exist; this antibody will detect all three.
Swissprot #:
Q9BXY4
User NOte:
Optimal dilutions for each application to be determined by the researcher.