DNAJC13 Antibody
| Code | Size | Price |
|---|
| PSI-8109-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8109-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
DNAJC13 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8
Application Note:
DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
DNAJC13, also known as receptor-mediated endocytosis 8 (RME8), is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1). It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2). DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3).
Background References:
- Girard M, Poupon V, Blondeau F, et al. The DnaJ-domain protein RME-8 functions in endosomal trafficking. J. Biol. Chem. 2005; 280:40135-43.
- Fujibayashi A, Taguchi T, Misaki R, et al. Human RME-8 is involved in membrane trafficking through early endosomes. Cell Struct. Funct. 2008; 33:35-50.
- Girard M and McPherson PS. RME-8 regulates trafficking of the epidermal growth factor receptor. FEBS Lett. 2008; 582:961-6.
Buffer:
DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.
The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
The immunogen is located within amino acids 1940 - 1990 of DNAJC13.
NCBI Gene ID #:
23317
NCBI Official Name:
DnaJ (Hsp40) homolog, subfamily C, member 13
NCBI Official Symbol:
DNAJC13
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 249 kDa
Observed: 249 kDa
Observed: 249 kDa
Protein Accession #:
NP_056083
Protein GI Number:
112421122
Purification:
DNAJC13 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.
Swissprot #:
O75165
User NOte:
Optimal dilutions for each application to be determined by the researcher.