DTX4 Antibody

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ProSci
Product Code: PSI-8057
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8057-0.02mg0.02mg£150.00
Quantity:
PSI-8057-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.
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Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.
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Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.
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Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.
Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.
Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
Deltex 4 E3 ubiquitin ligase, RNF155
Application Note:
DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).
Background References:
  • Kishi N, Tang Z, Maeda Y, et al. Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. Int. J. Dev. Neurosci. 2001; 19:21-35.
  • Cui J, Li Y, Zhu L, et al. NLRP4 negatively regulated type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4. Nat. Immunol. 2012; 13:387-95.
Buffer:
DTX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4.

The immunogen is located within amino acids 230 - 280 of DTX4.
NCBI Gene ID #:
23220
NCBI Official Name:
deltex 4, E3 ubiquitin ligase
NCBI Official Symbol:
DTX4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 68 kDa

Observed: 68 kDa
Protein Accession #:
NP_055992
Protein GI Number:
148237498
Purification:
DTX4 antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience,Innate Immunity
SPECIFICITY:
DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.
Swissprot #:
Q9Y2E6
User NOte:
Optimal dilutions for each application to be determined by the researcher.