DNAJA4 Antibody

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ProSci
Product Code: PSI-8141
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8141-0.02mg0.02mg£150.00
Quantity:
PSI-8141-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
DNAJA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of DNAJA4 in human colon tissue lysate with DNAJA4 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
DnaJ homolog subfamily A member 4, MST104, MSTP104, PRO1472
Application Note:
DNAJA4 antibody can be used for detection of DNAJA4 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
The DNAJA4 protein is the mammalian homolog of the chaperone HSP40 (1) and acts as a co-chaperone with the heat shock protein HSP70 (2). DNAJA4 is highly expressed in heart and testis, accounting for approximately 1% of total protein in mouse heart (2). DNAJA4 is also regulated by the sterol regulatory element binding transcription factor 2 (SREBF2) and plays a role in the synthesis of cholesterol (3).
Background References:
  • Ohtsuka K and Hata M. Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. Cell Stress Chaperones 2000; 5:98-112.
  • Abdul KM, Terada K, Gotoh T, et al. Characterization and functional analysis of a heart-enriched DnaJ / Hsp40 homolog dj4/DjA4. Cell Stress Chaperones 2002; 7:156-66.
  • Robichon C, Varret M, Le Liepvre X, et al. DnajA4 is a SREBP-regulated chaperone involved in the cholesterol biosynthesis pathway. Biochim. Biophys. Acta 2006; 1761:1107-13.
Buffer:
DNAJA4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (87%)
Immunogen:
DNAJA4 antibody was raised against a 15 amino acid peptide near the center of human DNAJA4.

The immunogen is located within amino acids 210 - 260 of DNAJA4.
NCBI Gene ID #:
55466
NCBI Official Name:
DnaJ homolog subfamily A member 4
NCBI Official Symbol:
DNAJA4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 41, 44, 47 kDa

Observed: 40 kDa
Protein Accession #:
NP_061072
Protein GI Number:
194328758
Purification:
DNAJA4 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
DNAJA4 antibody is human, mouse, and rat reactive. At least three isoforms of DNAJA4 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q8WW22
User NOte:
Optimal dilutions for each application to be determined by the researcher.