AJAP1 Antibody
| Code | Size | Price |
|---|
| PSI-8163-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8163-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
AJAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Adherens junctions associated protein 1, Membrane protein shrew-1, MOT8, SHREW1
Application Note:
AJAP1 antibody can be used for detection of AJAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
The Adherens Junction Associated Protein 1 (AJAP1) plays a key role in cell adhesion and cell migration (1). It is a transmembrane protein found on the basolateral surface of epithelium and expressed by multiple types of epithelium (1,2). AJAP1 interacts with E-Cadherin and appears to promote the internalization of E-Cadherin upon growth factor stimulation (3). This facilitates the dissolution of adherens junctions with an increase in cell motility (3,4).
Background References:
- Zeng L, Fee BE, Rivas MV, et al. Adherens junctional associated protein-1: a novel 1p36 tumor suppressor candidate in gliomas (Review). Int. J. Oncol. 2014; 45:13-7.
- Han L, Zhang KL, Zhang JX, et al. AJAP1 is dysregulated at an early stage of gliomagenesis and suppresses invasion through cytoskeleton reorganization. CNS Neurosci. Ther. 2014; 20:429-37.
- Gross JC, Schreiner A, Engels K, et al. E-cadherin surface levels in epithelial growth factor-stimulated cells depend on adherens junction protein shrew-1. Mol. Biol. Cell 2009; 20:3598-607.
- Lin N, Di C, Bortoff K, et al. Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma. Mol. Cancer Res. 2012; 10:208-17.
Buffer:
AJAP1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
AJAP1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human AJAP1.
The immunogen is located within amino acids 290 - 340 of AJAP1.
The immunogen is located within amino acids 290 - 340 of AJAP1.
NCBI Gene ID #:
55966
NCBI Official Name:
adherens junctions associated protein 1
NCBI Official Symbol:
AJAP1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 45 kDa
Observed: 48 kDa
Observed: 48 kDa
Protein Accession #:
NP_061324
Protein GI Number:
9055278
Purification:
AJAP1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Cancer
SPECIFICITY:
AJAP1 antibody is human, mouse and rat reactive.
Swissprot #:
Q9UKB5
User NOte:
Optimal dilutions for each application to be determined by the researcher.