C2CD3 Antibody

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ProSci
Product Code: PSI-8169
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8169-0.02mg0.02mg£150.00
Quantity:
PSI-8169-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
C2CD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of C2CD3 in EL4 cell lysate with C2CD3 antibody at (A) 1 and (B) 2 μg/ml.
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Western blot analysis of C2CD3 in EL4 cell lysate with C2CD3 antibody at (A) 1 and (B) 2 μg/ml.

Documents

Further Information

Additional Names:
C2 calcium-dependent domain containing 3, C2 domain-containing protein 3
Application Note:
C2CD3 antibody can be used for detection of C2CD3 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
C2CD3, a putative calcium-dependent lipid-binding protein, is an essential regulator of intracellular transduction of the Hedgehog signal (1). It is localized at the basal body of cilia, mediates calcium-dependent vesicular transport and/or recruitment of proteins and regulates cilium biogenesis by promoting the assembly of centriolar distal appendages critical for docking ciliary vesicles and recruiting other essential ciliogenic proteins (2,3). C2CD3 directly promotes centriole elongation and OFD1 acts as a negative regulator of C2CD3 (4,5).
Background References:
  • Hoover AN, Wynkoop A, Zeng H, et al. C2cd3 is required for cilia formation and Hedgehog signaling in mouse. Development 2008; 135:4049-58.
  • Ye X, Zeng H, Ning G, et al. C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals. Proc. Natl. Acad. Sci. USA 2014; 111:2164-9.
  • Thauvin-Robinet C, Lee JS, Lopez E, et al. The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation. Nat. Genet. 2014; 46:905-11.
  • Balestra FR, Strnad P, Fluckiger I, et al. Discovering regulators of centriole biogenesis through siRNA-based functional genomics in human cells. Dev. Cell 2013; 25:555-71.
Buffer:
C2CD3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
C2CD3 antibody was raised against an 18 amino acid peptide near the center of human C2CD3.

The immunogen is located within amino acids 1490 - 1540 of C2CD3.
NCBI Gene ID #:
26005
NCBI Official Name:
C2 calcium-dependent domain containing 3
NCBI Official Symbol:
C2CD3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 216, 259 kDa

Observed: 255 kDa
Protein Accession #:
NP_001273506
Protein GI Number:
148596944
Purification:
C2CD3 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Signal Transduction
SPECIFICITY:
C2CD3 antibody is human, mouse and rat reactive.
Swissprot #:
Q4AC94
User NOte:
Optimal dilutions for each application to be determined by the researcher.