PACS1 Antibody
| Code | Size | Price |
|---|
| PSI-8187-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8187-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PACS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Phosphofurin acidic cluster sorting protein 1, PACS-1, MRD17
Application Note:
PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
The phosphofurin acidic cluster sorting protein 1 (PACS1), belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1,2). Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3). PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4).
Background References:
- Wan L, Molloy SS, Thomas L, et al. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. Cell 1998; 94:205-16.
- Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
- Schuurs-Hoeijmakers JH, Oh EC, Vissers LE, et al. Recurrent de novo mutations in PACS1 cause defective cranial-neural-crest migration and define a recognizable intellectual-disability syndrome. Am. J. Hum. Genet. 2012; 91:1122-7.
- Dikeakos JD, Thomas L, Kwon G, et al. An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS2 on endosomes to down-regulate MHC-I. Mol. Biol. Cell 2012; 23:2184-97.
Buffer:
PACS1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PACS1 antibody was raised against a 17 amino acid peptide near the center of human PACS1.
The immunogen is located within amino acids 430 - 480 of PACS1.
The immunogen is located within amino acids 430 - 480 of PACS1.
NCBI Gene ID #:
55690
NCBI Official Name:
Phosphofurin acidic cluster sorting protein 1
NCBI Official Symbol:
PACS1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 106 kDa
Observed: 105 kDa
Observed: 105 kDa
Protein Accession #:
NP_060496
Protein GI Number:
30089916
Purification:
PACS1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Infectious Disease,Stem Cell
SPECIFICITY:
PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.
Swissprot #:
Q6VY07
User NOte:
Optimal dilutions for each application to be determined by the researcher.