PACS2 Antibody
| Code | Size | Price |
|---|
| PSI-8189-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8189-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PACS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Phosphofurin acidic cluster sorting protein 2, PACS-2, PACS1-like protein, PACS1L
Application Note:
PACS2 antibody can be used for detection of PACS2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
PACS2 (phosphofurin acidic cluster sorting protein-2), PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS family (1,2). It is expressed in a broad range of tissues with highest expression in skeletal muscle, brain, heart, testis and pancreas (2,). PACS2 interacts with Polycystin-2 and BID and functions as a sorting protein that regulates mitochondria-ER communication and is thought to be involved in ion channel trafficking, specifically direct cluster-containing ion channels to distinct subcellular compartments (3-5).
Background References:
- Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
- Brasacchio D, Noori T, House C, et al. A functional genomics screen identifies PCAF and ADA3 as regulators of human granzyme B-mediated apoptosis and Bid cleavage. Cell Death Differ. 2014; 21:748-60.
- Werneburg NW, Bronk SF, Guicciardi ME, et al. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein-induced lysosomal translocation of proapoptotic effectors is mediated by phosphofurin acidic cluster sorting protein-2 (PACS-2). J. Biol. Chem. 2012; 287:24427-37.
- You H and Thomas G. A homeostatic switch in PACS-2 links membrane traffic to TRAIL-induced apoptosis. Cell Cycle 2009; 8:2679-80.
Buffer:
PACS2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PACS2 antibody was raised against a 17 amino acid peptide near the center of human PACS2.
The immunogen is located within amino acids 330 - 380 of PACS2.
The immunogen is located within amino acids 330 - 380 of PACS2.
NCBI Gene ID #:
23241
NCBI Official Name:
Phosphofurin acidic cluster sorting protein 2
NCBI Official Symbol:
PACS2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 98 kDa
Observed: 95 kDa
Observed: 95 kDa
Protein Accession #:
NP_056012
Protein GI Number:
155029546
Purification:
PACS2 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
PACS2 antibody is human, mouse and rat reactive. At least three isoforms of PACS2 are known to exist; this antibody will detect all three. PACS2 antibody is predicted to not cross-react with PACS1.
Swissprot #:
Q86VP3
User NOte:
Optimal dilutions for each application to be determined by the researcher.