MAGEA4 Antibody
| Code | Size | Price |
|---|
| PSI-8191-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8191-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
MAGEA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Melanoma antigen family A4, MAGE4, MAGE-41, MAGE4A, MAGE-4 antigen, MAGE4B, MAGE-X2, MAGE-X2 antigen, Melanoma-associated antigen 4
Application Note:
MAGEA4 antibody can be used for detection of MAGEA4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
MAGEA4 is a member of the melanoma-associated antigen family that consists of a number of antigens recognized by cytotoxic T lymphocytes (1). MAGEA4 may serve as a target for antitumoral vaccination and may play a role in embryonal development and tumor transformation or aspects of tumor progression. It is expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma and have been implicated in some hereditary disorders, such as dyskeratosis congenital (2-4).
Background References:
- Bhan S, Negi SS, Shao C, et al. BORIS binding to the promoters of cancer testis antigens, MAGEA2, MAGEA3, and MAGEA4, is associated with their transcriptional activation in lung cancer. Clin. Cancer Res. 2011; 17:4267-76.
- Bhan S, Chuang A, Negi SS, et al. MAGEA4 induces growth in normal oral keratinocytes by inhibiting growth arrest and apoptosis. Oncol. Rep. 2012; 28:1498-502.
- Saito T, Wada H, Yamasaki M, et al. High expression of MAGE-A4 and MHC class I antigens in tumor cells and induction of MAGE-A4 immune responses are prognostic markers of CHP-MAGE-A4 cancer vaccine. Vaccine, epub. 2014; S0264-410X(14)01217-1.
- Zhang QM, He SJ, Shen N, et al. Overexpression of MAGE-D4 in colorectal cancer is a potentially prognostic biomarker and immunotherapy target. Int. J. Clin. Exp. Pathol. 2014; 7:3918-27.
Buffer:
MAGEA4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MAGEA4 antibody was raised against a 16 amino acid peptide near the amino terminus of human MAGEA4.
The immunogen is located within the first 50 amino acids of MAGEA4.
The immunogen is located within the first 50 amino acids of MAGEA4.
Molecular Weight Explanation:
multiple independent MAGEA4 antibodies also show a band at ~45 kDa
NCBI Gene ID #:
4103
NCBI Official Name:
melanoma antigen family A, 4
NCBI Official Symbol:
MAGEA4
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 35 kDa
Observed: 45 kDa
Observed: 45 kDa
Protein Accession #:
NP_001011550
Protein GI Number:
58530871
Purification:
MAGEA4 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Cancer
SPECIFICITY:
MAGEA4 antibody is human specific. At least four isoforms of MAGEA4 are known to exist.
Swissprot #:
P43358
User NOte:
Optimal dilutions for each application to be determined by the researcher.