PDIA1 Antibody
| Code | Size | Price |
|---|
| PSI-8213-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8213-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
PDIA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Protein disulfide isomerase family A member 1, DSI, GIT, PDI, PHDB, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta
Application Note:
PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2). PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3). Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4).
Background References:
- Pihlajaniemi T, Helaakoski T, Tasanen K, et al. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987; 6L:643-9.
- Walmsley AR, Batten MR, Lad U, et al. Intracellular retention of procollagen within the endoplasmic reticulum is mediated by prolyl 4-hydroxylase. J. Biol. Chem. 1999; 274:14884-92.
- Koivu J, Myllyla R, Halaakoski T, et al. A singel polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J. Biol. Chem. 1987; 262:6447-9.
- Kivirikko KI and Myllyharju J. Prolyl 4-hydroxylases and their protein disulfide isomerase subunit. Matrix Biol. 1998; 16:357-68.
Buffer:
PDIA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%), Chicken: (88%)
Immunogen:
PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1.
The immunogen is located within amino acids 190 - 240 of PDIA1.
The immunogen is located within amino acids 190 - 240 of PDIA1.
NCBI Gene ID #:
5034
NCBI Official Name:
prolyl 4-hydroxylase, beta polypeptid
NCBI Official Symbol:
P4HB
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 54 kDa
Observed: 54 kDa
Observed: 54 kDa
Protein Accession #:
NP_000909
Protein GI Number:
20070125
Purification:
PDIA1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
PDIA1 antibody is human, mouse and rat reactive.
Swissprot #:
P07237
User NOte:
Optimal dilutions for each application to be determined by the researcher.