MKRN3 Antibody

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ProSci
Product Code: PSI-8145
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8145-0.02mg0.02mg£150.00
Quantity:
PSI-8145-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
MKRN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
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Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.
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Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Makorin ring finger 3, CPPB2, D15S9, RNF63, ZFP127, ZNF127
Application Note:
MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Makorin ring finger 3 (MKRN3) protein contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. The MKRN3 gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome (1), but a deletion of the gene does not (2). A deficiency of MKRN3 has been shown to cause central precocious puberty in humans (3).
Background References:
  • Jong MT, Gray TA, Ji Y, et al. A novel imprinted gene, encoding a RING zinc-finger protein, a overlapping antisense transcript in the Prader-Willi syndrome critical region. Hum. Mol. Genet. 1999; 8:783-93.
  • Kanber D, Giltay J, Wieczorek D, et al. A paternal deletion of MKRN3, MAGEL2 and NDN does not result in Prader-Willi syndrome. Eur. J. Hum. Genet. 2009; 17:582-90.
  • Abreu AP, Dauber A, Macedo DB, et al. Central precocious puberty caused by mutations in the imprinting gene MKRN3. N. Engl. J. Med. 2013; 368:2467-75.
Buffer:
MKRN3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MKRN3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MKRN3.

The immunogen is located within amino acids 400 - 450 of MKRN3.
NCBI Gene ID #:
7681
NCBI Official Name:
makorin ring finger protein 3
NCBI Official Symbol:
MKRN3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 55 kDa

Observed: 55 kDa
Protein Accession #:
NP_005655
Protein GI Number:
5032243
Purification:
MKRN3 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family.
Swissprot #:
Q6NSB6
User NOte:
Optimal dilutions for each application to be determined by the researcher.