ADRM1 Antibody

Contact us
ProSci
Product Code: PSI-8119
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8119-0.02mg0.02mg£150.00
Quantity:
PSI-8119-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
ADRM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

1 / 2
Western blot analysis of ADRM1 in EL cell lysate with ADRM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunohistochemistry of ADRM1 in human spleen tissue with ADRM1 antibody at 5 μg/mL.
❮❯

Western blot analysis of ADRM1 in EL cell lysate with ADRM1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ADRM1 in human spleen tissue with ADRM1 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
Adhesion regulating molecule 1, ARM1, Gp110, hRpn13, Proteasomal ubiquitin receptor ADRM1, Proteasome regulatory particle non-ATPase 13, Rpn13
Application Note:
ADRM1 antibody can be used for detection of ADRM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
The adhesion-regulating molecule 1 (ADRM1) is thought to be involved in protein recruitment and cell adhesion and functions as a proteasomal ubiquitin receptor (1). ADRM1 promotes the activity of UCH-L5 and plays a role in lymphocyte homing. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37, perhaps serving as a mechanism to accelerate ubiquitin chain disassembly (2,3). ADRM1 expression is induced by IFN-gamma in some cancer cell lines and its expression is upregulated in other metastatic cells, suggesting a role in carcinogenesis (4,5).
Background References:
  • Husnjak K, Elsasser S, Zhang N, et al. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008; 453:481-8.
  • Yao T, Song L, Xu W, et al. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nat. Cell Biol. 2006; 8:994-1002.
  • Qiu XB, Ouyang SY, Li CJ, et al. hRpn13/ADRM1/GP110 is a novel proteasome subunit that binds the deubiquitinating enzyme, UCH37. EMBO J. 2006; 25:5742-53.
  • Al-Shami A, Jhaver KG, Vogel P, et al. Regulators of the proteasome pathway, Uch37 and Rpn13, play distinct roles in mouse development. PLoS One 2010; 5:e13654.
Buffer:
ADRM1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
ADRM1 antibody was raised against a 19 amino acid peptide near the center of human ADRM1.

The immunogen is located within amino acids 210 - 260 of ADRM1.
NCBI Gene ID #:
11047
NCBI Official Name:
adhesion regulating molecule 1
NCBI Official Symbol:
ADRM1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 45 kDa

Observed: 48 kDa
Protein Accession #:
NP_783163
Protein GI Number:
28373194
Purification:
ADRM1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Homeostasis
SPECIFICITY:
ADRM1 antibody is human, mouse and rat reactive. At least two isoforms of ADRM1 are know to exist; this antibody will detect both isoforms.
Swissprot #:
Q16186
User NOte:
Optimal dilutions for each application to be determined by the researcher.