MYH3 Antibody

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ProSci
Product Code: PSI-8087
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8087-0.02mg0.02mg£150.00
Quantity:
PSI-8087-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
MYH3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.
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Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.
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Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.
Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.

Documents

Further Information

Additional Names:
Myosin heavy chain 3, Myosin-3, Myosin heavy chain skeletal muscle embryonic, HEMHC, MYHC-EMB, MYHSE1, SMHCE
Application Note:
MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).
Background References:
  • Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030.
  • Lagrutta AA, McCarthy JG, Scherczinger CA, et al. Identification and developmental expression of a novel embryonic myosin heavy-chain gene in chicken. DNA 1989; 8:39-50.
  • Rutland CS, Polo-Parada L, Ehler E, et al. Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart. Development 2011; 138:3955-66.
  • Toydemir RM, Rutherford A, Whitby FG, et al. Mutations in embryonic myosin heavy chain (MYH3) cause Freeman-Sheldon syndrome and Sheldon-Hall syndrome. Nat. Genet. 2006; 38:561-5.
Buffer:
MYH3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3.

The immunogen is located within amino acids 30 - 80 of MYH3.
NCBI Gene ID #:
4621
NCBI Official Name:
myosin, heavy chain 3, skeletal muscle, embryonic
NCBI Official Symbol:
MYH3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 240 kDa

Observed: 245 kDa
Protein Accession #:
NP_002461
Protein GI Number:
98986453
Purification:
MYH3 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Swissprot #:
P11055
User NOte:
Optimal dilutions for each application to be determined by the researcher.