GLIS2 Antibody
| Code | Size | Price |
|---|
| PSI-8091-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8091-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
GLIS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
GLIS family zinc finger 2, GLI-similar 2, neuronal krueppel-like protein, NKL, NPHP7
Application Note:
GLIS2 antibody can be used for detection of GLIS2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
GLIS2, also known as neuronal Krueppel-like, is a 524 amino acid protein that belongs to the GLI C2H2-type zinc-finger protein family (1). GLIS2 can act either as a transcription repressor or as a transcription activator and may be involved in neuron differentiation (1,2). GLIS2 is expressed at high levels in kidney and at low levels in heart, lung and placenta. Mutations of GLIS2 have been suggested to be associated with development of progressive chronic kidney disease with characteristics resembling nephronophthisis (3,4).
Background References:
- Vasanth S, ZeRuth G, Kang HS, et al. Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 2011; 286:4749-59.
- Hosking CR, Ulloa F, Hogan C, et al. The transcriptional repressor Glis2 is a novel binding partner for p120 catenin. Mol. Biol. Cell 2007; 18:1918-27.
- Kim YS, Kang HS, Herbert R, et al. Kruppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal functions. Mol. Cell Biol. 2008; 28:2358-67.
- Attanasio M, Uhlenhaut NH, Sousa VH, et al. Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nat. Genet. 2007; 39:1018-24.
Buffer:
GLIS2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GLIS2 antibody was raised against an 18 amino acid peptide near the center of human GLIS2.
The immunogen is located within amino acids 260 - 310 of GLIS2.
The immunogen is located within amino acids 260 - 310 of GLIS2.
NCBI Gene ID #:
84662
NCBI Official Name:
GLIS family zinc finger 2
NCBI Official Symbol:
GLIS2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58 kDa
Observed: 60 kDa
Observed: 60 kDa
Protein Accession #:
NP_115964
Protein GI Number:
110431364
Purification:
GLIS2 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell,Neuroscience
SPECIFICITY:
GLIS2 antibody is human, mouse and rat reactive.
Swissprot #:
Q9BZE0
User NOte:
Optimal dilutions for each application to be determined by the researcher.