ADORA2B Antibody
| Code | Size | Price |
|---|
| PSI-8111-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8111-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
ADORA2B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Adenosine receptor A2b, ADORA2
Application Note:
ADORA2B antibody can be used for detection of ADORA2B by Western blot at 0.25 ? 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
The adenosine receptor ADORA2B is a member of the G protein-coupled receptor superfamily and is an integral membrane protein that stimulates adenylate cyclase activity in the presence of adenosine (1,2). Extracellular adenosine triggers a potent anti-inflammatory response that is mediated in part by ADORA2B including the stimulation of IL-10 production (3-5). Activation of ADORA2B can also enhance the abundance of regulatory T cells (Tregs), a class of cells that work to constrain inflammation (6).
Background References:
- Pierce KD, Furlong TJ, Selbie LA, et al. Molecular cloning and expression of an adenosine A2b receptor from human brain. Biochem. Biophys. Res. Commun. 1992; 187:86-93.
- Thimm D, Schiedel AC, Sherbiny FF, et al. Ligand-specific binding and activation of the human adenosine A(2B) receptor. Biochem. 2013; 52:726-40.
- Blackburn MR, Vance CO, Morschl E, et al. Adenosine receptors and inflammation. Handb. Exp. Pharmacol. 2009; 215-69.
- Grenz A, Homann D, Eltzschig HK. Extracellular adenosine: a safety signal that dampens hypoxia-induced inflammation during ischemia. Antioxid. Redox Signal. 2011; 15:2221-34.
Buffer:
ADORA2B antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (80%)
Immunogen:
ADORA2B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADORA2B.
The immunogen is located within the last 50 amino acids of ADORA2B.
The immunogen is located within the last 50 amino acids of ADORA2B.
NCBI Gene ID #:
136
NCBI Official Name:
adenosine A2b receptor
NCBI Official Symbol:
ADORA2B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 37 kDa
Observed: 41 kDa
Observed: 41 kDa
Protein Accession #:
NP_000667
Protein GI Number:
4501951
Purification:
ADORA2B antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology,Signal Transduction
SPECIFICITY:
ADORA2B antibody is human, mouse and rat reactive. At least two isoforms of ADORA2B are known to exist; this antibody will detect both isoforms. ADORA2B antibody is predicted to not cross-react with ADORA2A.
Swissprot #:
P29275
User NOte:
Optimal dilutions for each application to be determined by the researcher.