NOSTRIN Antibody
| Code | Size | Price |
|---|
| PSI-8039-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8039-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
NOSTRIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Nitric oxide synthase traffic inducer, eNOS-trafficking inducer, Nitric oxide synthase trafficker, BM247 homolog, DaIP2
Application Note:
NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).
Background References:
- Zimmermann K, Opitz N, Dedio J, et al. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. USA 2002; 99:17167-72.
- Icking A, Matt S, Opitz N, et al. NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS. J. Cell Sci. 2005; 118:5059-69.
- Schilling K, Opitz N, Wiesenthal A, et al. Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN. Mol. Biol. Cell 2006; 17:3870-80.
- Icking A, Schilling K, Wiesenthal A, et al. FCH/Cdc15 domain determines distinct subcellular localization of NOSTRIN. FEBS Lett. 2006; 580:223-8.
Buffer:
NOSTRIN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN.
The immunogen is located within amino acids 430 - 480 of NOSTRIN.
The immunogen is located within amino acids 430 - 480 of NOSTRIN.
NCBI Gene ID #:
115677
NCBI Official Name:
nitric oxide synthase trafficking
NCBI Official Symbol:
NOSTRIN
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 47, 53, 56, 62 kDa
Observed: 68 kDa
Observed: 68 kDa
Protein Accession #:
NP_443178
Protein GI Number:
284172479
Purification:
NOSTRIN antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms.
Swissprot #:
Q8IVI9
User NOte:
Optimal dilutions for each application to be determined by the researcher.