PPM1D Antibody
| Code | Size | Price |
|---|
| PSI-8043-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8043-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
PPM1D antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Protein phosphatase Mg2+/Mn2+ dependent 1D, p53-induced protein phosphatase 1, PP2C-DELTA, Protein phosphatase 1D, WIP1
Application Note:
PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D), a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1,2). Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2,3). PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4,5).
Background References:
- Fiscella M, Zhang H, Fan S, et al. Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. Proc. Natl. Acad. Sci. USA. 1997; 94:6048-53.
- Zhu YH and Bulavin DV. Wip1-dependent signaling pathways in health and diseases. Prog. Mol. Biol. Transl. Sci. 2012; 106:307-25.
- Lu X, Nguyen TA, Moon SH, et al. The type 2C phosphatase Wip1: an oncogenic regulator of tumor suppressor and DNA damage response pathways. Cancer Metastasis Rev. 2008; 27:123-35.
- Liang C, Guo E, Lu S, et al. Over-expression of wild-type p53-induced phosphatase 1 confers poor prognosis of patients with gliomas. Brain Res. 2012; 1444:65-75.
Buffer:
PPM1D antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D.
The immunogen is located within the last 50 amino acids of PPM1D.
The immunogen is located within the last 50 amino acids of PPM1D.
NCBI Gene ID #:
8493
NCBI Official Name:
Protein phosphatase Mg2+/Mn2+ dependent 1D
NCBI Official Symbol:
PPM1D
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 67 kDa
Observed: 68 kDa
Observed: 68 kDa
Protein Accession #:
NP_003611
Protein GI Number:
4505997
Purification:
PPM1D antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.
Swissprot #:
Q8IVR6
User NOte:
Optimal dilutions for each application to be determined by the researcher.