CRBN Antibody

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ProSci
Product Code: PSI-8115
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8115-0.02mg0.02mg£150.00
Quantity:
PSI-8115-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
CRBN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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<strong>Figure 1 Immunofluorescence Validation of CRBN in HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with 8115 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
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<strong>Figure 3 WB Validation in Human Cells </strong><br>Loading: 15 μg of cell lysate. Antibodies: CRBN 8115, 1 μg/mL,1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of mouse tissue lysate. Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of rat tissue lysate. Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 1 Immunofluorescence Validation of CRBN in HeLa Cells</strong><br>Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with 8115 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
<strong>Figure 3 WB Validation in Human Cells </strong><br>Loading: 15 μg of cell lysate. Antibodies: CRBN 8115, 1 μg/mL,1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of mouse tissue lysate. Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of rat tissue lysate. Antibodies: CRBN 8115, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A
Application Note:
CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
CRBN, a member of the Lon protease protein family, plays a role in brain development (1). It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2). CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3,4).
Background References:
  • Xin W, Xiaohua N, Peilin C, et al. Primary function analysis of human mental retardation related gene CRBN. Mol. Biol. Rep. 2008; 35:251-6.
  • Gandhi AK, Kang J, Havens CG, et al. Immunomodulatory agents lenalidomide and pomalidomide co-stimulate T cells by inducing degradation of T cell repressors Ikaros and Aiolos via modulation of the E3 ubiquitin ligase complex CRL4(CRBN). Br. J. Haematol. 2014; 164:811-21.
  • Liu J, Ye J, Zou X, et al. CRL4A (CRBN) E3 ubiquitin ligase restricts BK channel activity and prevents epileptogenesis. Nat. Commun. 2014; 5:3924.
  • Heintel D, Rocci A, Ludwig H, et al. High expression of cereblon (CRBN) is associated with improved clinical response in patients with multiple myeloma treated with lenalidomide and dexamethasone. Br. J. Haematol. 2013; 161:695-700.
Buffer:
CRBN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.

The immunogen is located within the last 50 amino acids of CRBN.
NCBI Gene ID #:
51185
NCBI Official Name:
cereblon
NCBI Official Symbol:
CRBN
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 49 kDa

Observed: 54 kDa
Protein Accession #:
NP_057386
Protein GI Number:
39545580
Purification:
CRBN antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience,Stem Cell
SPECIFICITY:
CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Swissprot #:
Q96SW2
User NOte:
Optimal dilutions for each application to be determined by the researcher.