CTHRC1 Antibody
| Code | Size | Price |
|---|
| PSI-8101-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-8101-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
CTHRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Documents
Further Information
Additional Names:
Collagen triple helix repeat-containing protein 1
Application Note:
CTHRC1 antibody can be used for detection of CTHRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Collagen triple helix repeat-containing protein (CTHRC1) is a glycosylated, secreted protein that is transiently expressed in the arterial wall in response to injury where it is thought to contribute to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration (1). CTHRC1 is highly expressed multiple human cancers, including pancreatic cancer where it plays a significant role in the progression and metastasis of the disease by regulating migration and adhesion of tumors cells (2). CTHRC1 overexpression can also be used as an independent prognostic marker in gastric cancer (3). Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma (4).
Background References:
- Pyagay P, Heroult M, Wang Q, et al. Collagen triple helix repeat containing 1, a novel secreted protein in injured and diseased arteries, inhibits collagen expression and promotes cell migration. Circ. Res. 2005; 96:261-8.
- Park EH, Kim S, Jo JY, et al. Collagen triple helix repeat containing-1 promotes pancreatic cancer progression by regulating migration and adhesion of tumor cells. Carcinogenesis 2013; 34:694-702.
- Gu L, Liu L, Zhong L, et al. Cthrc1 overexpresion is an independent prognostic marker in gastric cancer. Hum. Pathol. 2014; 45:1031-8.
- Orloff M, Peterson C, He X, et al. Germline mutations in MSR1, ASCC1, and CTHRC1 in patients with Barrett esophagus and esophageal adenocarcinoma. JAMA 2011; 306:410-9.
Buffer:
CTHRC1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CTHRC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CTHRC1.
The immunogen is located within the first 50 amino acids of CTHRC1.
The immunogen is located within the first 50 amino acids of CTHRC1.
NCBI Gene ID #:
115908
NCBI Official Name:
Collagen triple helix repeat-containing protein 1
NCBI Official Symbol:
CTHRC1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 27 kDa
Observed: 27 kDa
Observed: 27 kDa
Protein Accession #:
NP_612464
Protein GI Number:
19923989
Purification:
CTHRC1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
CTHRC1 antibody is human, mouse and rat reactive. At least two isoforms of CTHRC1 are known to exist; this antibody will detect only the larger isoform.
Swissprot #:
Q96CG8
User NOte:
Optimal dilutions for each application to be determined by the researcher.