NAP1L5 Antibody

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ProSci
Product Code: PSI-8147
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-8147-0.02mg0.02mg£150.00
Quantity:
PSI-8147-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
NAP1L5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Images

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Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.
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Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.
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Western blot analysis of NAP1L5 in EL4 cell lysate with NAP1L5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 5 μg/mL.
Immunofluorescence of NAP1L5 in mouse brain tissue with NAP1L5 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Nucleosome assembly protein 1-like 5, DRLM, down-regulated in liver malignancy
Application Note:
NAP1L5 antibody can be used for detection of NAP1L5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
The nucleosome assembly protein 1-like 5 (NAP1L5) protein, also known as DRLM, was initially identified as a possible tumor suppressor gene in hepatocellular carcinoma (1). The NAP1L5 gene is located within the intron of Herc3, and unlike Herc3, is imprinted and highly expressed in regions of the adult mouse brain (2).
Background References:
  • Harada H, Nagai H, Ezura Y, et al. Down-regulation of a novel gene, DRLM, in human liver malignancy from 4q22 that encodes a NAP-like protein. Gene 2002; 296:171-7.
  • Davies W, Smith RJ, Kelsey G, et al. Expression patterns of the novel imprinted genes Nap1L5 and Peg13 and their non-imprinted host genes in the adult mouse brain. Gene Expr. Patterns 2004; 4:741-7.
Buffer:
NAP1L5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
NAP1L5 antibody was raised against an 18 amino acid peptide near the center of human NAP1L5.

The immunogen is located within amino acids 40 - 90 of NAP1L5.
NCBI Gene ID #:
266812
NCBI Official Name:
nucleosome assembly protein 1-like 5
NCBI Official Symbol:
NAP1L5
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 20 kDa (17kDa in mouse)

Observed: 15 kDa
Protein Accession #:
NP_715638
Protein GI Number:
24371268
Purification:
NAP1L5 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer
SPECIFICITY:
NAP1L5 antibody is human, mouse and rat reactive.
Swissprot #:
Q96NT1
User NOte:
Optimal dilutions for each application to be determined by the researcher.